rabbit anti cd206 polyclonal antibody  (Elabscience Biotechnology)

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A) Quantitative analysis of iNOS protein levels; (B) Quantitative analysis of CD16/32 protein levels; (C) Quantitative analysis of Arg-1 protein levels; (D) Quantitative analysis of CD206 protein levels. (E-F) Flow cytometry (FCM) analysis of the proportion of CD16/32 + cells in the different experimental groups; (G-H) FCM analysis of the proportion of CD206 + cells in the different experimental groups. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Flow Cytometry, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A-B) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD16/32 (red) and DAPI (green); Scale bar = 100 μm. (C-D) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD206 (red) and DAPI (green); Scale bar = 100 μm. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Double Immunostaining, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A) Quantitative analysis of iNOS protein levels; (B) Quantitative analysis of CD16/32 protein levels; (C) Quantitative analysis of Arg-1 protein levels; (D) Quantitative analysis of CD206 protein levels. (E-F) Flow cytometry (FCM) analysis of the proportion of CD16/32 + cells in the different experimental groups; (G-H) FCM analysis of the proportion of CD206 + cells in the different experimental groups. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Flow Cytometry, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A-B) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD16/32 (red) and DAPI (green); Scale bar = 100 μm. (C-D) Representative images of double immunostaining and quantitative analysis of BV2 microglial M1 polarization, with CD206 (red) and DAPI (green); Scale bar = 100 μm. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Double Immunostaining, Comparison

Journal: PLOS One

Article Title: TNFAIP3 alleviates cerebral ischemia-reperfusion injury by inhibiting M1 microglia polarization via deubiquitination of RACK1

doi: 10.1371/journal.pone.0337601

Figure Lengend Snippet: (A) Quantitative analysis of iNOS protein levels; (B) Quantitative analysis of CD16/32 protein levels; (C) Quantitative analysis of Arg-1 protein levels; (D) Quantitative analysis of CD206 protein levels. (E-F) Flow cytometry (FCM) analysis of the proportion of CD16/32 + cells in the different experimental groups; (G-H) FCM analysis of the proportion of CD206 + cells in the different experimental groups. Data are presented as the mean ± SD from replicate experiments (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. The endpoints of the line segment indicate the comparison between the two groups under consideration.

Article Snippet: Subsequently, cells were incubated with 3% BSA for 30 min at 37°C to block non-specific binding, and then exposed to primary antibodies at 4°C overnight, including anti-CD206 rabbit polyclonal antibody (1:100; cat. no. DF4149; Affinity Biosciences) and anti-CD16/32 (1:500; cat. no. ab223200; Abcam).

Techniques: Flow Cytometry, Comparison